The Negative Effect Of Cisplatine On The Reproductive System Of Albino Rats And The Preventive Role Of Silybum Marianum Seed Extract And Vitamin C
Keywords:
Cisplatin, Silybum Marianum, Vit C, Testosterone, LH; FSH
Abstract
This "study aims to test the efficacy of Silybum marianum and vitamin C against CP toxicity on hormone secretion and testicular function in sperm production. Thirty male albino mice aged between (98-112) days were used. The animals were divided into (6 groups) (five per group) and the animals were treated with cisplatin, vitamin C and alcoholic extract of Silybum marianum for one month as follows: control group (i) animals given distilled water (1 ml), infected group (ii) injected with CP at a concentration of (2 mg/kg) subperitoneally once a week, group (iii) injected with vitamin C at a concentration of (100 mg/kg), group (iii) injected with alcoholic extract of Silybum marianum at a concentration of (200) mg/kg, group (iiii) injected subperitoneally with CP at a concentration of (2 mg/kg) weekly for 4 weeks and injected with vitamin C daily at a concentration of (100 mg/kg) at one time until the end of the experiment, group (iiii) injected subperitoneally with CP at a concentration of (2 mg/kg) were given a dose of alcoholic extract of Silybum marianum At a concentration of (200 mg/kg) daily at the same time weekly for 4 weeks until the end of the experiment. After the end of the dose period, the rats were anesthetized, then blood was drawn by cardiac stab and placed in a gel tube and placed in a centrifuge for 20 minutes / 5000 rpm. The serum was stored at a temperature of 20 °C in an Eppendorf tube until the date of testing the hormones LH, FSH and testosterone. After that, the animals were dissected, the testicles were removed and placed in formalin (10%) for fixation for three days, then washed with water and stored in 70% alcohol. The results showed the following: Figure 1, 2, 3 show that the CP-treated group showed a significant decrease in the concentration of LH, FST and testosterone hormones compared to the healthy group, while treatment with vitamin C, treatment with Silybum marianum alcoholic extract, treatment with vitamin C and cisplatin, treatment with Silybum marianum alcoholic extract and CP led to a significant increase in the concentration of LH, FST and testosterone hormones compared to the control group. The results of the histological sections of the testicles showed the presence of colloidal material inside the tubules and incomplete maturation of spermatozoa in the cisplatin-treated group. In the vitamin C-treated group and the Silybum marianum extract-treated group, the seminiferous tubules appeared normal and the stages of spermatogenesis were observed with the observation of Leydig cells. Histological sections of the CP and vitamin C treated group and the CP and Silybum marianum extract treated group showed that the seminiferous tubules were in normal shape, and the stages of spermatogenesis were observed, with Leydig cells" observed.
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Elateeq, Y. Sun, W. Nxumalo, A.M.M. (2020). Gabr Biotechnological production of silymarin in Silybum marianum L.: A review Biocatalysis and Agricultural Biotechnology, 29 (July).
Fatehi, D., Anjomshoa, M., Mohammadi, M., Seify, M., & Rostamzadeh, A. (2018). Biological effects of cell-phone radiofrequency waves exposure on fertilization in mice; an in vivo and in vitro study. Middle East Fertility Society Journal, 23(2), 148-153.
Filobbos, S., Amin, N., Yacoub, M., & Abd El_Hakim, K. R. (2020). Possible protective effect of melatonin on cisplatin-induced testicular toxicity in adult albino rats. A histological and immunohistochemical study. Egyptian Journal of Histology, 43(3), 891-901.
Garcia, C. C. (2021). Toxicological Impact of Three Pharmacological Therapeutics on Human Sperm (Master's thesis, Universidade de Aveiro (Portugal).
Ge, R. S., Li, X., & Wang, Y. (2021). Leydig cell and spermatogenesis. Molecular Mechanisms in Spermatogenesis, 111-129.
Hamid, A. K., Ahmed, M. A., & Tayawi, H. M. (2018). Silymarin effect as an antioxidant to improve damages induced by CCl4 on some characteristics of male rats reproductive system. Tikrit Journal of Pure Science, 23(2), 60-65.
Hassen, M. T., Mohamed, H. K., Montaser, M. M., El-Sharnouby, M. E., Awad, N., & Ebiya, R. A. (2021). Molecular, immunomodulatory, and histopathological role of mesenchymal stem cells and beetroot extract on cisplatin induced testicular damage in albino rats. Animals, 11(4), 1142.
He, L., He, T., Farrar, S., Ji, L., Liu, T., & Ma, X. (2017). Antioxidants maintain cellular redox homeostasis by elimination of reactive oxygen species. Cellular Physiology and Biochemistry, 44(2), 532-553.
Houda, A., Nyaz, S., Sobhy, B. M., Bosilah, A. H., Romeo, M., Michael, J. P., & Eid, H. M. (2021). Seminiferous tubules and spermatogenesis. Male Reproductive Anatomy.
Ijaz, M. U., Tahir, A., Ahmed, H., Ashraf, A., Ahmedah, H. T., Muntean, L., ... & Irimie, M. (2022). Chemoprotective effect of vitexin against cisplatin-induced biochemical, spermatological, steroidogenic, hormonal, apoptotic and histopathological damages in the testes of Sprague-Dawley rats. Saudi Pharmaceutical Journal, 30(5), 519-526.
Javeed, A., Ahmed, M., Sajid, A. R., Sikandar, A., Aslam, M., Hassan, T. U., ... & Li, C. (2022). Comparative Assessment of Phytoconstituents, Antioxidant Activity and Chemical Analysis of Different Parts of Milk Thistle Silybum marianum L. Molecules, 27(9), 2641.
Kamali, F. S., Shahrooz, R., Najafi, G. R., & Razi, M. (2020). Ameliorative effect of crocin on sperm parameters and in vitro fertilization in mice under oxidative stress induced by paraquat. International Journal of Fertility & Sterility, 13(4), 307.
Kata, F. S. (2013). Effect of Cisplatin drug on sperm characterizes, spermatogenesis and sex hormones levels of male mice Mus musculus L. J Basrah Res (Sciences), 39(2A), 112-24.
Keshta, A. T., Fathallah, A. M., Attia, Y. A., Salem, E. A., & Watad, S. H. (2023). Ameliorative effect of selenium nanoparticles on testicular toxicity induced by cisplatin in adult male rats. Food and Chemical Toxicology, 179, 113979.
Khamis, T., Hegazy, A. A., El-Fatah, S. S. A., Abdelfattah, E. R., Abdelfattah, M. M. M., Fericean, L. M., & Arisha, A. H. (2023). Hesperidin mitigates cyclophosphamide-induced testicular dysfunction via altering the hypothalamic pituitary gonadal axis and testicular steroidogenesis, inflammation, and apoptosis in male rats. Pharmaceuticals, 16(2), 301.
Klimant, E., Wright, H., Rubin, D., Seely, D., and Markman, M. (2018). Intravenous vitamin C in the supportive care of cancer patients: a review and rational approach. Curr. Oncol. 25, 139–148. doi: 10.3747/co.25.3790
Kothari, R., Waghmare, S., & Choudhari, A. R. (2017). Role of ascorbic acid in male fertility and its relation with free testosterone. Int. J. Contemp. Med. Res, 4, 2454-7379.
Lamia, S. S., Emran, T., Rikta, J. K., Chowdhury, N. I., Sarker, M., Jain, P., ... & Reza, H. M. (2021). Coenzyme Q10 and silymarin reduce CCl4-induced oxidative stress and liver and kidney injury in ovariectomized rats—implications for protective therapy in chronic liver and kidney diseases. Pathophysiology, 28(1), 50-63.
Latacela, G. A., Ramaiah, P., Patra, I., Jalil, A. T., Gupta, R., Madaminov, F. A., ... & Farhood, B. (2023). The radioprotective potentials of silymarin/silibinin against radiotherapy-induced toxicities: A systematic review of clinical and experimental studies. Current Medicinal Chemistry, 30(33), 3775-3797.
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Published
2024-12-14
How to Cite
Daham, H., & Matni, A. (2024). The Negative Effect Of Cisplatine On The Reproductive System Of Albino Rats And The Preventive Role Of Silybum Marianum Seed Extract And Vitamin C. Central Asian Journal of Theoretical and Applied Science, 5(8), 703-713. Retrieved from https://cajotas.centralasianstudies.org/index.php/CAJOTAS/article/view/1524
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