The Negative Effect Of Cisplatine On The Reproductive System Of Albino Rats And The Preventive Role Of Silybum Marianum Seed Extract And Vitamin C

This "study aims to test the efficacy of Silybum marianum and vitamin C against CP toxicity on hormone secretion and testicular function in sperm production. Thirty male albino mice aged between (98-112) days were used. The animals were divided into (6 groups) (five per group) and the animals were treated with cisplatin, vitamin C and alcoholic extract of Silybum marianum for one month as follows: control group (i) animals given distilled water (1 ml), infected group (ii) injected with CP at a concentration of (2 mg/kg) subperitoneally once a week, group (iii) injected with vitamin C at a concentration of (100 mg/kg), group (iii) injected with alcoholic extract of Silybum marianum at a concentration of (200) mg/kg, group (iiii) injected subperitoneally with CP at a concentration of (2 mg/kg) weekly for 4 weeks and injected with vitamin C daily at a concentration of (100 mg/kg) at one time until the end of the experiment, group (iiii) injected subperitoneally with CP at a concentration of (2 mg/kg) were given a dose of alcoholic extract of Silybum marianum At a concentration of (200 mg/kg) daily at the same time weekly for 4 weeks until the end of the experiment. After the end of the dose period, the rats were anesthetized, then blood was drawn by cardiac stab and placed in a gel tube and placed in a centrifuge for 20 minutes / 5000 rpm. The serum was stored at a temperature of 20 °C in an Eppendorf tube until the date of testing the hormones LH, FSH and testosterone. After that, the animals were dissected, the testicles were removed and placed in formalin (10%) for fixation for three days, then washed with water and stored in 70% alcohol. The results showed the following: Figure 1, 2, 3 show that the CP-treated group showed a significant decrease in the concentration of LH, FST and testosterone hormones compared to the healthy group, while treatment with vitamin C, treatment with Silybum marianum alcoholic extract, treatment with vitamin C and cisplatin, treatment with Silybum marianum alcoholic extract and CP led to a significant increase in the concentration of LH, FST and testosterone hormones compared to the control group. The results of the histological sections of the testicles showed the presence of colloidal material inside the tubules and incomplete maturation of spermatozoa in the cisplatin-treated group. In the vitamin C-treated group and the Silybum marianum extract-treated group, the seminiferous tubules appeared normal and the stages of spermatogenesis were observed with the observation of Leydig cells. Histological sections of the CP and vitamin C treated group and the CP and Silybum marianum extract treated group showed that the seminiferous tubules were in normal shape, and the stages of spermatogenesis were observed, with Leydig cells" observed.